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目前国际上测定血清IgG含量多采用在玻璃板上的琼脂里进行放射状扩散法,此法最早为Mancini等人发明,故又称Mancini技术。其原理是以IgG为抗原,滴入合有抗IgG抗体的凝胶板上的小圆孔中,抗原抗体反应在凝胶中形成特异的沉淀,此沉淀围绕小孔呈圆形环,在抗原、抗体量足够并比例合适的情况下,抗原抗体复合物能形成肉眼可见的沉淀环,抗原、抗体浓度在一定比例时,
The current international determination of serum IgG content in the agar on the glass with radial diffusion method, this method was first invented by Mancini et al., Also known as Mancini technology. The principle is to use IgG as an antigen, dropped into a small hole in the gel plate with the anti-IgG antibody, and the antigen-antibody reaction forms a specific precipitate in the gel. The precipitate forms a circular ring around the pore, In the case of sufficient quantity and proportion, the antigen-antibody complex can form a precipitation ring which is visible to the naked eye. When the antibody concentration is in a certain proportion,