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目的:对贵阳市2010年手足口病流行期间的手足口病患儿进行病原学及流行特征分析。方法:采用Real-TimePCR法检测307例手足口病患儿标本样本中的肠道病毒通用型(EV)、肠道病毒71型(EV71)、肠道病毒科萨奇A16型(CoxA16)3种核酸,将扩增产物测序获得的序列与GenBank中肠道病毒核苷酸序列进行比对,确定毒株血清型别。结果:肠道病毒通用型(EV)阳性率为68.27%,CA16阳性率为6.73%,EV71阳性率为13.46%。重症患者EV71的检出率为18.60%,CoxA16的检出率为4.65%,其他肠道病毒为57.55%,4株其它肠道病毒经鉴定属于CA10型。结论:手足口病病原复杂,2010年贵阳地区HFMD的主要病原为其他肠道病毒和EV71,存在一定比例的其他未知病原体(CoxA10)。
Objective: To analyze the etiological and epidemiological characteristics of hand, foot and mouth disease in Guiyang during the HFMD epidemic in 2010. Methods: Real-Time PCR was used to detect three types of enterovirus (EV), enterovirus 71 (EV71) and enterovirus CoxA16 in 307 samples of HFMD. Nucleic acid, the amplified product was sequenced sequence obtained with the GenBank enterovirus nucleotide sequence comparison to determine the strain serotypes. Results: The positive rate of EV was 68.27%, the positive rate of CA16 was 6.73%, and the positive rate of EV71 was 13.46%. In critically ill patients, the detection rate of EV71 was 18.60%, that of CoxA16 was 4.65%, and that of other enteroviruses was 57.55%. Four other enteroviruses were identified as CA10. Conclusion: The pathogens of hand-foot-mouth disease are complex. The major pathogens of HFMD in Guiyang in 2010 are other enterovirus and EV71, and some other unknown pathogens (CoxA10) exist in some areas.