论文部分内容阅读
目的 观察体外蒿甲醚与血红素伍用对日本血吸虫磷酸甘油酸激酶（PGK）和丙酮酸激酶（PK）的影响。方法 以 4～5周龄的血吸虫,置于含蒿甲醚和/或血红素的培素液内培养24h后,测定虫体的PGK和PK酶活力。结果体外,蒿甲醚（50μmol/L）或血红素（50μmol/L）对血吸虫的PGK和PK酶活力无影响,但两者伍用可引起两种酶的活力明显下降。结论 蒿甲醚对血吸虫PGK和PK的影响具有血红素依赖性。
Objective To observe the effects of in vitro application of artemether and heme on the phosphoglycerate kinase (PGK) and pyruvate kinase (PK) of Schistosoma japonicum. METHODS: Schistosoma japonicum (4 ~ 5 weeks old) was cultured in culture medium containing artemether and / or heme for 24 hours to determine the activity of PGK and PK. Results In vitro, artemether (50μmol / L) or heme (50μmol / L) had no effect on the activity of PGK and PK in Schistosoma japonicum. Conclusion The effect of artemether on PGK and PK of schistosomiasis is heme-dependent.