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用HPLC法同时测定食品中的四种B族维生素(B_1,B_2,B_5,B_6)。实验用0.4mol/L盐酸,120℃、15psi水解样品。分析以μBondapakC18(300mm×3.9mm)为色谱柱,甲醇:重蒸水:庚烷磺酸(PIC-B_7)=30:68:2(V:V:V)为流动相,选择紫外254nm,对B_1、B_2和B_5以及荧光(EX295nm、EM395nm)对B_6的双通道检测,可得到很好的分离图形。B_1、B_2、B_5和B_6的线性范围与相关系数各为10～50ng(r=0.998),5～40ng(r=0.999),5～40ng(r=0.993),5～50ng(r=0.999),最小检出限各为5、5、2、2ng。回收率各为91.3％～102％,103％～105％,92％～100％和99％～100％。与参比实验相比,所得食物中B族维生素含量差异不大,并与食物成分表公布的数据十分接近。
Simultaneous Determination of Four B Vitamins (B_1, B_2, B_5, B_6) in Food by. Experiments with 0.4mol / L hydrochloric acid, 120 ℃, 15psi hydrolysis samples. The mobile phase consisted of μBondapak C18 (300 mm × 3.9 mm) column and methanol: distilled water: heptane sulfonic acid (PIC-B_7) = 30: 68: 2 B_1, B_2 and B_5 and fluorescence (EX295nm, EM395nm) pairs of B_6 dual-channel detection, get a good separation of graphics. The linear ranges and correlation coefficients of B_1, B_2, B_5 and B_6 were 10-50ng (r = 0.998), 5-40ng (r = 0.999), 5-40ng (r = 0.993) and 5-50ng (r = 0.999) , The minimum detection limit for each 5,5,2,2 ng. The recoveries were 91.3% ~ 102%, 103% ~ 105%, 92% ~ 100% and 99% ~ 100%, respectively. Compared with the reference experiment, the content of B vitamins in the obtained food has little difference and is close to the data published in the food composition table.