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标准氧化还原电位 E~(0′)和电子转移化学计量数 n 的测定是生物氧化还原体系研究中的一个重要方面.由于许多生物分子会在电极上吸附,有些蛋白分子的氧化还原中心位于蛋白结构的内部,它们同电极的异相电子转移过程很不可逆或非常缓慢,因而难于用常规的电化学方法进行 E_(0′)和 n 值的测量。近年来薄层电化学技术的发展为这方面的研究打开了方便之门,本文简要介绍其原理及应用。
The determination of the standard redox potential E ~ (0 ’) and the electron transfer stoichiometry n is an important aspect in the study of biological redox systems. Since many biomolecules adsorb on the electrodes, the redox center of some protein molecules is located at the protein Inside the structure, their heterogeneous electron transfer process with the electrode is very irreversible or very slow, making it difficult to measure E_ (0 ’) and n values by conventional electrochemical methods. In recent years, the development of thin-layer electrochemical technology has opened the door to the research in this field. This article briefly introduces its principle and application.