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UVR8(UV resistance locus 8)是目前唯一被描述的植物紫外光B(UV-B)特异受体,能感受环境中的UV-B,从而调控植物生长发育进程。本研究以甜樱桃‘红灯’果实为试验材料,利用RT-PCR技术,获得甜樱桃UVR8基因c DNA全长序列。该序列包含一个长1 329 bp的开放阅读框,编码442个氨基酸,相对分子质量为47.80 k D,将该基因命名为Pac UVR8并提交Gen Bank数据库,收录号为(KX671127)。氨基酸保守域分析表明,Pac UVR8基因编码的蛋白包含7个RCC1结构域,该氨基酸序列与其他植物序列相似性在78%~98%之间。同时,利用荧光定量PCR分析UVR8基因在3种不同颜色的樱桃果实生长过程的表达情况,其中‘黑樱桃’和‘红灯’随着果实成熟,果皮颜色逐渐变红,UVR8表达量逐渐上升;而黄色品种‘佐藤锦’在成熟时期基因表达量表现为先增长后减少,在转色期的20 d表达量最高。本研究为甜樱桃光受体对光应答的分子机理及解释甜樱桃着色分子机制提供了一定的理论依据。
UVR8 (UV resistance locus 8) is the only plant ultraviolet light B (UV-B) specific receptor that can sense the environment of UV-B, thereby regulating plant growth and development. In this study, the sweet cherry “red light” fruit as the experimental material, using the RT-PCR technology, sweet cherry UVR8 gene c DNA full-length sequence. The sequence contains a 1 329 bp open reading frame encoding 442 amino acids with a relative molecular mass of 47.80 kD. The gene was named Pac UVR8 and submitted to Gen Bank database under accession number (KX671127). Amino acid conserved domain analysis showed that the protein encoded by Pac UVR8 gene contains seven RCC1 domains, which share 78% ~ 98% identity with other plant sequences. At the same time, the expression of UVR8 gene in three different color cherry fruit was analyzed by fluorescence quantitative PCR. The color of peel became red with the ripening of “black cherry” and “red light” The yellow variety ’Satojin’ showed the first increase and then the decrease in the gene expression at maturity, and the highest expression was reached 20 days after the color change. This study provided the theoretical basis for the molecular mechanism of light response of sweet cherry photoreceptor and the molecular mechanism of sweet cherry coloring.