三尖杉酯碱脂质体的制剂研究

• 作 者: 汤丽娟 翁帼英
• 机 构: 南京药物研究所,南京药物研究所 中国医学科学院药物研究所
• 出 处: 药学学报
• 发表日期: 1985年06期
• 关键词: 包裹率 脂质体 水溶性药物 三尖杉酯碱 溶血试验 卵磷脂 磷脂双分子膜 蒸发法 三尖杉 制剂研究

本文探讨三尖杉酯碱脂质体的较佳制备方法。表明用转相蒸发法(REV)制备时影响三尖杉酯碱脂质体包裹率的因素与大多数水溶性药物脂质体的影响因素相同。提出并经实验证实,以稠厚型脂质体贮存可减少内包三尖杉酯碱的渗漏。实验还表明脂质体100℃30分钟灭菌和10℃以下贮存五个月,制品的结构、粒度和包裹率均无明显改变,溶血试验合格。但在40℃加速实验1至3个月制品产生溶血,此溶血与卵磷脂的氧化有明显相关性。

This article discusses the better preparation of harmalline liposomes. The results showed that the factors influencing the encapsulation efficiency of harringtonine liposomes by phase inversion evaporation (REV) were the same as those of most water-soluble drug liposomes. It is proposed and experimentally confirmed that the storage of thick liposomes can reduce the leakage of encapsulated harringtonine. Experiments also showed that the liposomes were sterilized at 100 ° C for 30 minutes and stored at 10 ° C for five months. No significant changes were found in the structure, particle size and encapsulation rate of the products, and the hemolysis test was qualified. However, 40 ℃ accelerated experiment 1 to 3 months products hemolysis, hemolysis and lecithin oxidation was significantly correlated.