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蛋白质和多肽是由各种氨基酸通过肽键相连而成,要阐明蛋白质的一级结构需先将其序列降解,然后测定依次降解出来的氨基酸。通常用Edman方法来降解蛋白质N-端氨基酸,使生成苯基海硫因氨基酸(简称PTH-氨基酸),而鉴定PTH-氨基酸常用的方法是气相色谱、薄层色谱和将降解生成的PTH-氨基酸再水解测定氨基酸的方法。近几年来,用高压液相色
Proteins and peptides are composed of a variety of amino acids linked by peptide bonds formed, to elucidate the primary structure of the protein must first degrade its sequence, and then determine the degradation of amino acids. The Edman method is commonly used to degrade the N-terminal amino acid of a protein to generate the phenylthiothionine amino acid (PTH-amino acid for short), and the commonly used methods for identifying PTH-amino acids are gas chromatography, thin layer chromatography and the degradation of PTH- Rehydrolysis method for the determination of amino acids. In recent years, high-pressure liquid color