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作者试用马来丝虫经口感染沙鼠。从实验室饲养的埃及伊蚊获得马来丝虫的第3期幼虫,感染6周龄的雄性沙鼠。每只沙鼠给予100条幼虫,其中经口灌入未麻醉的沙鼠3只,用苯巴比妥钠腹腔麻醉的7只;另以乙醚轻度麻醉的5只沙鼠,采用鼠蹊部皮下注射作为对照。于感染后第8周始,每周从沙鼠眶后静脉取20立方毫米血计数微丝蚴,至16～20周止,用乙醚将沙鼠杀死,从心腔取20立方毫米血标本计数微丝蚴,剖检时将各组织分别浸泡于pH7.2磷酸盐缓冲液碟中,将所有组织撕碎后在解剖显微镜下寻找成虫。
The authors try to infect male gerbils with gerbils orally. The third larva of the malayian filariasis was obtained from laboratory-housed Aedes aegypti and infected with 6-week-old male gerbils. Each larva was given 100 larvae, of which 3 rats were injected intraperitoneally with no anesthesia and 7 with intraperitoneal anesthesia with phenobarbital sodium. Five gerbils were anesthetized with diethyl ether and subcutaneously Injection as a control. At the beginning of the 8th week after infection, microfilariae of 20 cubic millimeters of blood were taken weekly from the gerbil retro-orbital venous and until 16 to 20 weeks, gerbils were killed with diethyl ether and 20 mm3 blood samples Microfilaria count, the anatomy were immersed in each tissue pH7.2 phosphate buffer dish, all the tissue shredded under a dissecting microscope looking for adults.