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目的:观察海人酸(KA)颞叶癫痫大鼠海马细胞内游离钙离子浓度、线粒体膜电位及线粒体酶活性和细胞能量代谢的改变,探讨线粒体功能障碍在海马神经元凋亡中的作用。方法:海人酸颞叶癫癎大鼠模型采用一侧海马注射海人酸制备。40只SD大鼠随机分为生理盐水对照组和KA组(6 h、1、3、7天),每组8只。检测各组海马细胞内游离钙离子浓度、线粒体膜电位、线粒体呼吸链复合物Ⅱ和Ⅳ以及Na~+-K~+-ATP酶活性、三磷酸腺苷(ATP)和二磷酸腺苷(ADP)的浓度。结果:KA组大鼠海马细胞内游离钙离子浓度显著增加,线粒体膜电位、线粒体呼吸链复合物Ⅱ和Ⅳ以及Na~+-K~+-ATP酶活性、ATP浓度、ATP/ADP比值显著降低,且随时间延长,其下降尤为显著。结论:KA大鼠海马存在能量代谢异常和线粒体功能障碍。
Objective: To observe the changes of intracellular free calcium concentration, mitochondrial membrane potential, mitochondrial enzyme activity and cellular energy metabolism in hippocampal slices of kainate (KA) temporal lobe epilepsy rats and to explore the role of mitochondrial dysfunction in apoptosis of hippocampal neurons. Methods: The rat model of kainic acid temporal lobe epilepsy was prepared by injecting kainate into the hippocampus. Forty SD rats were randomly divided into saline control group and KA group (6 h, 1, 3, 7 days), with 8 rats in each group. The concentrations of free calcium, mitochondrial membrane potential, mitochondrial respiratory chain complexes Ⅱ and Ⅳ and Na ~ + -K ~ + -ATPase activity, ATP and ADP in hippocampus of each group were measured. . Results: The concentration of intracellular free calcium in hippocampus of rats in KA group was significantly increased. The mitochondrial membrane potential, mitochondrial respiratory chain complex Ⅱ and Ⅳ, and Na ~ + -K ~ + -ATPase activity, ATP concentration and ATP / ADP were significantly decreased , And its decline is particularly significant over time. Conclusion: KA rats have abnormal energy metabolism and mitochondrial dysfunction in hippocampus.