论文部分内容阅读
目的研究不同临床类型慢性乙型肝炎患者外周血白细胞膜γ干扰素受体1(IFN-γR1)反应的差异性及其临床意义。方法采用流式细胞技术及夹心酶联免疫吸附法检测53例慢性乙型肝炎患者外周血细胞膜IFN-γR1表达及血清IFN-γ水平变化,并与血生物化学指标、肝组织病理变化进行相关性分析。结果慢性重型肝炎淋巴细胞膜IFN-γR1表达水平显著高于正常对照[(28.89％±11.77％)与(9.23％±1.30％)],两者差异有显著性(Z=3.988,P<0.05)。各组间单核细胞膜IFN-γR1表达水平变化差异无显著性。肝硬化、重型肝炎两者血清IFN-γ水平较正常对照,差异有显著性升高,肝硬化、重型肝炎IFN-γ水平组内差异较大,组间无差异。慢性乙型肝炎患者淋巴细胞膜IFN-γR1与总胆红素显著相关(r=0.575,P<0.01),与肝组织炎症活动度显著相关(r=0.621,P<0.01)。结论慢性乙型肝炎淋巴细胞通过上调膜IFN-γR1参与免疫病理。
Objective To study the difference and clinical significance of IFN-γR1 response in peripheral blood leukocytes from patients with different clinical types of chronic hepatitis B. Methods Flow cytometry and sandwich enzyme-linked immunosorbent assay were used to detect the expression of IFN-γR1 and the level of serum IFN-γ in peripheral blood of 53 patients with chronic hepatitis B. The correlations were also analyzed with the indexes of blood biochemistry and pathological changes of liver analysis. Results The level of IFN-γR1 expression in chronic severe hepatitis was significantly higher than that in controls (28.89% ± 11.77% vs 9.23% ± 1.30%, respectively). There was significant difference between the two groups (Z = 3.988, P <0.05). There was no significant difference in the expression level of IFN-γR1 in monocyte membrane between groups. Serum levels of IFN-γ in patients with cirrhosis and severe hepatitis were significantly higher than those in controls, but there was no significant difference among the groups of cirrhosis and severe hepatitis with IFN-γ. Lymphocyte membrane IFN-γR1 was significantly associated with total bilirubin in patients with chronic hepatitis B (r = 0.575, P <0.01), and significantly correlated with liver inflammation (r = 0.621, P <0.01). Conclusion Chronic hepatitis B lymphocytes play an important role in immunopathology by up-regulating membrane IFN-γR1.