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目的探讨Nogo-A蛋白在缺氧缺血性脑损伤新生鼠脑组织中的表达特点及黄芪对其表达的影响方法 7日龄Wistar新生大鼠72只,清洁级,雌雄不限,平均体重10-16g,随机分为假手术组,HIBD组,黄芪治疗组,每组24只。分离左侧颈总动脉并结扎,吸入92%N2+8%O2氮氧混合气体行缺氧处理2h,制成HIBD模型。假手术组只作分离左侧颈总动脉而不结扎,不作缺氧处理,不给与药物处理。黄芪治疗组于建模成功后立即及3h腹腔注射0.5ml/只,假手术组及HIBD组大鼠腹腔注射等量生理盐水,上述各组均于术后6h、12h、24h、72h随机抽取6只,于麻醉下处死取脑组织,HE染色观察脑组织病例变化,免疫组化法检测Nogo-A蛋白表达情况。结果与假手术组相比,HIBD组术后脑组织Nogo-A蛋白表达增高,但Nogo-A蛋白随各时间点有先上升后下降的趋势,差异有统计学意义(P<0.05),与HIBD组相比,黄芪治疗组各时间点Nogo-A蛋白,差异有统计学意义(P<0.05)。结论提示黄芪可下调缺氧缺血性损伤大鼠脑组织Nogo-A蛋白的表达,对神经元和神经纤维具有保护作用。
Objective To investigate the expression of Nogo-A protein in brain tissue of neonatal rats with hypoxic-ischemic brain damage and the effect of Astragalus membranaceus on its expression.Methods Seventy-two Wistar neonatal rats (7 days old) -16g, were randomly divided into sham operation group, HIBD group, Astragalus treatment group, 24 rats in each group. The left common carotid artery was isolated and ligated, and the HIBD model was made by inhalation of 92% N2 + 8% O2 N2O mixture for 2 hours. Sham operation group only for the left common carotid artery without ligation, not for hypoxia, not given drug treatment. Astragalus group was injected intraperitoneally with 0.5ml / rat immediately after modeling, and rats in sham operation group and HIBD group were given intraperitoneal injection of normal saline. The above groups were randomly selected 6h, 12h, 24h, 72h after operation Only under anesthesia, the brain tissues were sacrificed and the changes of brain tissues were observed by HE staining. The expression of Nogo-A protein was detected by immunohistochemistry. Results Compared with the sham group, the expression of Nogo-A protein in the brain tissue of HIBD group increased at first, then decreased at each time point, the difference was statistically significant (P <0.05) Compared with HIBD group, the Nogo-A protein in astragalus group at each time point showed significant difference (P <0.05). Conclusions Astragalus membranaceus can down-regulate the expression of Nogo-A protein in the brain tissue of rats with hypoxic-ischemic injury, which has a protective effect on neurons and nerve fibers.