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以抗条锈病基因 Ｙｒ１０的供体亲本 Ｍｏｒｏ作参照，用 ６个 Ｐｓｔ Ｉ端引物和 １０个 ＴａｑＩ端引物对抗条锈病基因Ｙｒ１０的近等基因系和轮回亲本Ａｖｏｃｅｔ Ｓ进行了ＡＦＬＰ分析，共扩增出约４２００条可分辨的带，其中５条为稳定的差异带．用Ｙｒ１０基因的供体亲本Ｍｏｒｏ和感病品种铭贤１６９杂交产生的Ｆ２代分离群体，对５个特异条带与目的基因的遗传连锁性进行了初步分析，发现特异条带ＰＴ０５０２与Ｙｒ１０基因连锁．将该片段进行克隆、测序，根据其序列设计了ＰＣＲ特异扩增用专化引物．经用１９５株Ｆ２代分离株进行遗传连锁性分析表明，用所设计的引物进行ＰＣＲ，其主要产物ＳＣ２００片段与抗条锈病基因Ｙｒ１０的连锁距离为０．５ｃＭ，可用于该基因的快速、有效、准确检测．
AFLP analysis was carried out by using six Pst I primer and ten TaqI primer pairs against the stripe rust resistance gene Yr10 near-isogenic line and the recurrent parent Avocet S, for the reference of the donor parent Moro of stripe rust resistance gene Yr10. Out of about 4,200 discernible bands, of which 5 are stable bands of difference. The genetic linkage between five specific bands and the target gene was analyzed by F2 population segregated by crossing the donor parent Moro of Yr10 with the susceptible cultivar Mingxian 169 and found that the specific band PT0502 was linked with the Yr10 gene . The fragment was cloned and sequenced, and specific primers for PCR specific amplification were designed according to its sequence. The genetic linkage analysis of 195 F2 progenies showed that the linkage between the SC200 fragment of the major product SC200 and the stripe rust resistance gene Yr10 was 0.5cM, which could be used for the rapid and effective , Accurate detection.