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常规食用菌一级菌种培养,均采用PDA培养基。我们尝试用成熟桑椹作培养基原料培养食用菌菌种,获得满意结果。 1 培养基制作 5月桑椹成熟季节采集呈黑紫色桑椹。配方:新鲜桑椹200g,琼脂18～20g,水1000mL。将桑椹加水煮沸5分钟,用双层纱布过滤挤压,滤液中加入(?)后再加热,待琼脂熔化后定容,分装灭菌。 2 接种培养 试验用菌种为平菇、金针菇、蘑菇、香菇四类共9种,接种至桑椹培养基中在25℃下培养。 3 培养结果 所有平菇和蘑菇培养1周菌丝长满培养基表面,尤其是平菇杂17气生菌丝相当旺盛。香菇、白金针菇培养10天后亦长满培养基表面,比用PDA生长迅速。在仅用桑椹单一营养基质时,以上几种食用菌均表现出生长旺势。因此,我们认为桑椹是一种良好的食用菌培养基。在试验中,接种的45管斜面中无一污染。之后又接种10管平菇杂17,在未加棉塞的情况
Conventional mushrooms a strain of culture, are used PDA medium. We try to use mature mulberry medium for the cultivation of edible fungi species, to obtain satisfactory results. 1 medium production May mulberry mature season collected black purple mulberry. Formula: fresh mulberry 200g, agar 18 ~ 20g, water 1000mL. The mulberry boil water for 5 minutes, with double gauze filter squeeze, the filtrate was added (?) And then heated until the agar melting volume, sterilization sterilization. 2 inoculation culture test for Pleurotus ostreatus, mushroom, mushrooms, mushrooms four kinds of a total of 9 species, inoculated into mulberry medium cultured at 25 ℃. 3 culture results All mushrooms and mushrooms cultivated for 1 week mycelium covered with medium surface, especially Pleurotus ostreatus 17 mycelium quite strong. Mushrooms, white mushroom growth after 10 days also covered with medium surface, faster than PDA growth. When using only a single nutrient substrate mulberry, the above edible fungi have shown growth potential. Therefore, we think mulberry is a good edible mushroom medium. In the experiment, there was no contamination in the inoculated 45-bevel. And then inoculated with 10 tubes Mushroom miscellaneous 17, in the absence of tampon situation