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以丙型肝炎病毒（ＨＣＶ）基因免疫的动物实验方法，将构建的ＨＣＶ基因重组体（ｐＣＤＨＣＶ１），采用不同方法免疫ＢＡＬＢ／ｃ小鼠，观察其诱发小鼠抗体水平变化。结果：ｐＣＤＨＣＶ１经肌肉注射３次免疫小鼠后，比１、２次免疫抗体水平要高；经灌胃、腹腔注射、皮下注射和肌肉注射不同途径免疫小鼠，以肌肉注射免疫效果最佳；不同剂量（１０μｇ、５０μｇ和１００μｇ）３次免疫小鼠，以１００μｇ剂量组诱发小鼠抗体反应最强；用普鲁卡因处理小鼠后再肌肉、皮下注射同剂量ｐＣＤＨＣＶ１，抗体水平均比直接注射同剂量ｐＣＤＨＣＶ１明显增高。
In the experiment of animal immunization with Hepatitis C virus (HCV) gene, BALB / c mice were immunized with different methods of recombinant HCV gene (HCVCD1) and the level of antibody induced in mice was observed. Results: The immunized mice with pCDHCV1 were immunized three times after intramuscular injection, which were higher than the first and second immunized mice. The mice immunized by gavage, intraperitoneal injection, subcutaneous injection and intramuscular injection were most effective in intramuscular injection (10μg, 50μg and 100μg) three times immunized mice, 100μg dose group induced the strongest antibody response in mice; mice treated with procaine and then muscle, subcutaneous injection of the same dose of pCD HC1 antibody Compared with the direct injection of the same dose of pCD HCV1 was significantly higher.