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神经元在体外的存活是衡量一种营养因子有无神经营养作用的重要指标之一。我们用人胚制备脊髓提取液,并用Centricon(Millipo-re)将粗提取液分成<10KD、10-30KD及>30KD三种组份,研究了粗提取液及这三种组份对体外培养中的脊髓神经元存活的影响,结果表明加粗提取液及<10KD的实验组比对照组活性要好,表现在线粒体中琥珀酸脱氢酶活性高(MTT法),神经元中NSE活性高(NSE-ELISA法)及细胞生长合成的总蛋白的量高等方面。但以<10KD组份对细胞的促活作用最强,与对照组相比有显著性差异。以上结果显示人胚脊髓中存在对脊髓神经元有促进存活的物质。
The survival of neurons in vitro is one of the important indicators to measure whether a nutritional factor has neurotrophic effects. We used human embryos to prepare spinal cord extract, and Centricon (Millipore re) the crude extract into <10KD, 10-30KD and> 30KD three components, the study of the crude extract and these three components of cultured in vitro The results showed that the crude extract and <10KD experimental group than the control group activity is better, showing mitochondrial succinate dehydrogenase activity (MTT method), high neuronal NSE activity (NSE- ELISA method) and the amount of total protein synthesized by cell growth. But with <10KD component of the most effective role in promoting cells, compared with the control group were significantly different. The above results show that there are substances that promote the survival of spinal cord neurons in human embryonic spinal cord.