【摘 要】
：
With the advantages of better mimicking the specificity of natural tissues,three-dimensional(3D)cell culture plays a major role in drug development,toxicity testing,and tissue engineering.However,exist-ing scaffolds or microcarriers for 3D cell culture ar
【机 构】
：
Department of Rheumatology and Immunology,Institute of Translational Medicine,Affiliated Drum Tower
论文部分内容阅读
With the advantages of better mimicking the specificity of natural tissues,three-dimensional(3D)cell culture plays a major role in drug development,toxicity testing,and tissue engineering.However,exist-ing scaffolds or microcarriers for 3D cell culture are often limited in size and show suboptimal perfor-mance in simulating the vascular complexes of living organisms.Therefore,we present a novel hierarchically inverse opal porous scaffold made via a simple microfluidic approach for promoting 3D cell co-culture techniques.The designed scaffold is constructed using a combined concept involving an emul-sion droplet template and inert polymer polymerization.This work demonstrates that the resultant scaf-folds ensure a sufficient supply of nutrients during cell culture,so as to achieve large-volume cell culture.In addition,by serially planting different cells in the scaffold,a 3D co-culture system of endothelial-cell-encapsulated hepatocytes can be developed for constructing certain functional tissues.It is also demon-strated that the use of the proposed scaffold for a co-culture system helps hepatocytes to maintain speci-fic in vivo functions.These hierarchically inverse opal scaffolds lay the foundation for 3D cell culture and even the construction of biomimetic tissues.
其他文献
目前,在相关规范及工程设计中,预应力高强混凝土管桩单桩竖向抗压承载力取值都是按不同岩土层的桩侧侧摩阻力及桩端的端承力相叠加,计算烦琐,准确度低.论文从能量守恒原理导出单桩竖向抗压承载力的简化公式,准确度满足工程需求,方便工程应用.
Dissolved oxygen(DO)is an important indicator of aquaculture,and its accurate forecasting can effec-tively improve the quality of aquatic products.In this paper,a new DO hybrid forecasting model is pro-posed that includes three stages:multi-factor analysi
为保证西部山区路网建设的顺利开展,需要对山区公路中小跨桥梁设计进行深入分析.论文从山区公路中小跨桥梁设计的特点及存在问题出发,论述了桥梁选型、上下部结构选择以及抗震方面的设计原则及优化措施,通过对工程实例分析,明确其设计要点.
超高层建筑对给排水系统与消防系统要求极高,导致其设计难度较大.为增强设计方案的科学性与合理性,保障超高层建筑给排水正常与消防安全,论文结合某超高层建筑项目,重点就给排水系统中的给水系统、排水系统、雨水系统和消防系统中的消防给水系统、自动喷水灭火系统以及气体灭火系统的设计展开研究.
以某特大型屋面及地面雨水收集系统项目为例,对特大型屋面与地面雨水收集系统进行概述,介绍了其雨水弃流过滤方式、雨水蓄水池的形式以及雨水处理工艺,探究了该系统的应用难点,并具体分析了有关雨水收集系统的应用,以保证特大型雨水收集系统的稳定运行.
针对目前高速公路建设中出现的路桥过渡段沉降问题,论文对其产生的原因以及沉降控制关键技术进行了分析,并依托实际工程,采用MidasCivil2020建立有限元模型,通过计算数值模拟和现场试验对新型路桥过渡段设计方法进行了研究.
在电力输送方面,防雷保护是需要重点关注的问题,尤其是在高海拔区域,由于海拔高、地形复杂、自然环境恶劣,且雷击风险较高,若线路防雷保护设计不当,会严重影响电力的正常输送.论文结合西藏地区500 kV输电线路建设项目,简要分析影响线路耐雷水平的因素,根据项目实际情况提出线路防雷保护设计的基本思路,并重点研究高海拔地区500kV输电线路防雷保护措施.
山区高速公路服务区一方面处于生态敏感区域,一方面受地形限制较大,因此,在设计上需要做到统筹兼顾,秉承可持续性设计理念.论文简要阐述了山区高速公路服务区可持续性设计的内涵及基本原则,重点探讨山区高速公路服务区可持续性设计的策略.
根据菏泽地区既有铁路现状,结合京雄商铁路与鲁南高铁的线路走向、功能定位及菏泽地区的规划布局,对高速铁路引入菏泽地区方案进行了系统的研究,分别比选了不同站位方案和站型方案,最终推荐采用线路走向较顺直、符合城市规划的菏泽东桶子河站位方案和投资较省、便于车站运输管理、工程界面清晰的线路别分场站型方案.
This paper presents a vision-based crack detection approach for concrete bridge decks using an integrated one-dimensional convolutional neural network(1D-CNN)and long short-term memory(LSTM)method in the image frequency domain.The so-called 1D-CNN-LSTM al