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目的:建立小鼠前列腺癌的血清学肿瘤标志物前列腺特异性蛋白94氨基酸(PSP94)检测系统。方法:建立小鼠血清PSP94竞争ELISA方法,并检测基因敲入小鼠前列腺癌模型小鼠的血清PSP94变化。结果:建立竞争ELISA方法,并以1ng/mL的灵敏度定量检测血清PSP94水平。野生型小鼠PSP94血清平均水平为49.8ng/mL,对于20周龄、40周龄和>40周龄的前列腺癌小鼠模型分别为150.9、835.5和774.8ng/mL。结论:首个小鼠类前列腺癌血清学标志物的建立将会极大地方便对人类前列腺癌的基础及临床前期研究。
Objective: To establish a detection system of prostate cancer specific protein 94 amino acid (PSP94) for mouse prostate cancer serum tumor marker. Methods: Mouse serum PSP94 competitive ELISA was established and the changes of serum PSP94 level in gene knock-in mouse model of prostate cancer were detected. Results: The competition ELISA method was established and the serum PSP94 level was quantitatively detected with the sensitivity of 1ng / mL. The mean level of PSP94 serum in wild-type mice was 49.8 ng / mL and were 150.9, 835.5 and 774.8 ng / mL for 20-week-old, 40-week-old and> 40-week-old prostate cancer mouse models, respectively. Conclusion: The establishment of the first mouse prostate cancer serological marker will greatly facilitate the basic and preclinical studies on human prostate cancer.