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本研究旨在观察臭氧应激不同时间,大鼠肺内血管活性肠肽(vasoactive intestinal peptide,VIP)含量和支气管粘蛋白总量的变化,分析两者之间的相关性。取64只Sprague-Dawley大鼠,臭氧应激不同时间后,用放射免疫方法检测肺组织匀浆VIP含量,过碘酸-希夫(periodic acid-Schiff,PAS)染色检测支气管粘蛋白总量的变化,分析两者之间的相关性。结果显示,呼吸道损伤初期,VIP变化不明显,支气管粘蛋白总量分泌增加,随着损伤加重,引起VIP代偿性分泌增加,支气管粘蛋白总量有所减少。随损伤时间的延长,损伤程度加重,肺内分泌VIP的阳性神经纤维及内分泌细胞数量减少,呼吸道对炎性介质的抗损伤作用下降,支气管粘蛋白总量逐渐增加。以上结果证实在气道高反应过程中,VIP分泌与支气管粘蛋白总量呈负相关。
The purpose of this study was to observe the changes of vasoactive intestinal peptide (VIP) and total bronchomucin in the lungs of rats exposed to ozone stress at different times and to analyze the correlation between the two. Totally 64 Sprague-Dawley rats were used. After different times of ozone stress, the content of VIP in lung homogenate was detected by radioimmunoassay, and the total amount of bronchial mucin was detected by periodic acid-Schiff (PAS) staining Change, analyze the correlation between the two. The results showed that in the early stage of respiratory tract injury, the changes of VIP were not obvious, the secretion of bronchial mucin increased, with the increase of injury, VIP compensatory secretion increased and the total amount of bronchial mucin decreased. With the extension of injury time, the degree of injury increased, the number of pulmonary endocrine VIP positive nerve fibers and endocrine cells decreased, the respiratory resistance to inflammatory mediators decreased, and the total amount of bronchial mucin increased. The above results confirmed that in the process of airway hyperresponsiveness, VIP secretion and the total amount of bronchial mucin was negatively correlated.