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目的研究萘甲异喹（NI）对大鼠心肌细胞外Ca(2+)内流的影响。方法：应用钙离子荧光指示剂Fura－2检测。结果：NI（3，10μmol·L(-1)）和维拉帕米（0．3μmol·L(－1)）对静息状态下心肌细胞内Ca(2+)浓度无影响，但可浓度依赖地抑制高钾（60mmol·L(-1)）和异丙肾上腺素（lμmol·L(－1)）引起的心肌细胞内Ca(2+)浓度的升高，抑制率分别为29％±6％、49％±9％和26％±6％、40％±8％；NI对两种激动剂作用的抑制百分率无明显差异，而维拉帕米对高钾作用的抑制大于对异丙肾上腺素作用的抑制。结论：NI对心肌细胞电压依赖性钙通道以及和β受体有关的钙通道有阻断作用，NI可能是非选择性钙通道阻滞剂。
Objective To investigate the effect of nalmelin on influx of extracellular Ca (2 +) in rat cardiac myocytes. Methods: Fura-2 was used to detect calcium ion fluorescence. Results NI (3,10 μmol·L -1) and verapamil (0.3 μmol·L -1) had no effect on intracellular Ca 2+ concentration at rest, Dependently inhibited the increase of intracellular Ca2 + concentration in myocardial cells induced by high potassium (60 mmol · L -1) and isoprenaline (L μmol·L -1), the inhibition rates were respectively 29% ± 6%, 49% ± 9% and 26% ± 6%, 40% ± 8%, respectively. There was no significant difference in the percent inhibition of the effect of NI on both agonists, while the inhibition of high potassium on verapamil was greater than that on isoprene Inhibition of adrenergic effects. Conclusion: NI can block the voltage-dependent calcium channels of cardiomyocytes and the β-adrenergic calcium channels. NI may be a non-selective calcium channel blocker.