ISOLATION, PURIFICATION AND BIOCHEMICAL CHARACTERIZATION OF E-RECEPTORS ON SURFACES OF PORCINE PERIP

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A report on the study of isolation, purification and bioehemical characterization of two glycoproteins, designated as Gp_1 and Op_2, with E-receptor activity from the surfaces of porcine peripheral lymphocytes is presented. The molecular weights of Gp_1 and Gp_2 have been found to be 58,000 and 29,000 Daltons, and the isoelectric points 6.8 and 5.5 respectively. Nterminal amino acid residues of Gp_1 are glycine and alanine, and that of Gp_2 is glycine. Monospecific rabbit anti-Gp_1 antiserum shown to inhibit E-rosette formation of porcine lymphocytes has been obtained. The present paper gives for the first time the evidence that the Ereceptor of porcine lymphocytes, Gp_1, appears to have cross antigenicity with that of human lymphocytes. Further studies of Gp_2 in detail and its relationship to Gp_1 will be published elsewhere. A report on the study of isolation, purification and bioemical characterization of two glycoproteins, designated as Gp_1 and Op_2, with E-receptor activity from the surfaces of porcine peripheral lymphocytes is. The molecular weights of Gp_1 and Gp_2 have been found to be 58,000 and 29,000 Daltons, and the isoelectric points 6.8 and 5.5 respectively. Nterminal amino acid residues of Gp_1 are glycine and alanine, and that of Gp_2 is glycine. Monospecific rabbit anti-Gp_1 antiserum shown to inhibit E-rosette formation of porcine lymphocytes has been obtained . The present paper gives for the first time the evidence that the Ereceptor of porcine lymphocytes, Gp_1, appears to have cross antigenicity with that of human lymphocytes. Further studies of Gp_2 in detail and its relationship to Gp_1 will be published elsewhere.
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