论文部分内容阅读
目的建立并评价人类巨细胞病毒PP65 Ig G抗体间接ELISA检测试剂。方法原核表达并纯化重组HCMV PP65332~561蛋白,包被酶标板,优化试剂检测条件,试制HCMV-Ig G间接ELISA试剂,分析试剂的精密度,并通过对100份样本的检测,与市售进口HCMV-Ig G间接ELISA试剂比较,分析了自制试剂的特异性、敏感性和符合率。结果试制的ELISA试剂批内和批间变异度均小于10%,与其他疱疹病毒感染血清均无交叉反应;与进口试剂的总符合率达到95%。结论该方法具有良好的特异性、敏感性,稳定性和可重复性,为HCMV病毒感染的流行病学调查提供了新的备选方法。
Objective To establish and evaluate human cytomegalovirus PP65 Ig G antibody indirect ELISA detection reagent. Methods The recombinant HCMV PP65332 ~ 561 protein was expressed in prokaryotic cells and purified by enzyme-linked immunosorbent assay (ELISA). The ELISA kit was used to test HCMV-Ig G indirect ELISA reagent. The precision of the reagent was analyzed. Through the detection of 100 samples, Imported HCMV-Ig G indirect ELISA reagent comparison, analysis of the specificity of self-made reagents, sensitivity and compliance rate. Results The intra-assay and inter-assay variation of ELISA reagents were all less than 10%, which showed no cross-reaction with other herpes virus-infected sera. The total coincidence rate with imported reagents reached 95%. Conclusion The method has good specificity, sensitivity, stability and repeatability, and provides a new alternative method for epidemiological investigation of HCMV virus infection.