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目的研究紫外线(UV)致弱日本血吸虫尾蚴诱导C57BL/6小鼠的免疫保护作用。方法分别观察不同UV强度(300、400和500μW/cm2照射的日本血吸虫尾蚴经腹部皮肤免疫)、不同免疫剂量(8、24和300条UV致弱尾蚴经腹部皮肤免疫)、不同免疫位点(300条UV致弱尾蚴经腹部和耳廓皮肤免疫)和不同免疫次数(100条UV致弱尾蚴经腹部皮肤免疫3次)诱导C57BL/6小鼠抗血吸虫攻击感染(40条正常尾蚴经腹部皮肤感染)的保护力。同时观察免疫后小鼠的体液免疫应答变化。结果300、400和500μW/cm2UV照射的日本血吸虫尾蚴免疫C57BL/6小鼠诱导产生的减虫率分别为2.72%、11.37%和10.38%;8、24和300条致弱尾蚴免疫小鼠诱导产生的减虫率分别为38.67%、7.54%和16.36%;300条致弱尾蚴经腹部和耳廓皮肤免疫诱导小鼠产生的减虫率分别为16.36%和16.14%;100条致弱尾蚴免疫3次,诱导小鼠产生减虫率为4.88%。对300条UV照射尾蚴免疫后小鼠的体液免疫应答动态观察显示,与感染对照组相比,免疫组血清中可溶性成虫抗原(SWA)和可溶性虫卵抗原(SEA)特异的IgG于免疫后2周开始升高,正常尾蚴抗原(SCA)特异的IgG于免疫1周后开始升高,SWA和SCA特异的IgG随免疫次数的增加而升高。结论UV致弱日本血吸虫尾蚴免疫C57BL/6小鼠能诱导其产生高水平的体液免疫应答,但保护力水平较低,提示C57BL/6小鼠为对UV致弱日本血吸虫尾蚴的低应答品系。
Objective To study the protective effect of ultraviolet (UV) on C57BL / 6 mice induced by cercariae of Schistosoma japonicum. Methods Different doses of UV radiation (300, 400 and 500μW / cm2 of cercariae of Schistosoma japonicum through the skin of the abdomen), different immunological doses (8, 24 and 300 immunostimulants of the irradiated cercariae of the abdomen), different immunological sites 300 C57BL / 6 mice were immunized with C57BL / 6 mouse anti-schistosomiasis (immunization of 40 normal cercariae via abdominal skin with 300 UV-irradiated cercariae via the abdomen and auricle skin) and different times of immunization (100 UV- Infection) protection. At the same time, the changes of humoral immune response in mice after immunization were observed. Results The worm reduction rates of C57BL / 6 mice immunized with cercaria of Schistosoma japonicum at doses of 300, 400 and 500μW / cm2 were 2.72%, 11.37% and 10.38%, respectively. 8, 24 and 300 mice immunized with cercariae were induced The worm reduction rates of mice induced by immunization of the abdomen and the auricle of 300 attenuated cercariae were 16.36% and 16.14%, respectively; and that of 100 attenuated cercariae coccidiosis was 38.67%, 7.54% and 16.36% Second, the induced mice produced worm reduction rate was 4.88%. The dynamic observation of the humoral immune response in mice immunized with 300 UV-irradiated cercariae showed that compared with the infected control group, the serum IgG of SWA and SEA in the immunized group was significantly lower than that of the immunized group Weekly began to rise, normal cercariae antigen (SCA) -specific IgG began to rise after 1 week of immunization, SWA and SCA-specific IgG increased with the increase in the number of immunizations. Conclusion C57BL / 6 mice immunized with UV-attenuated Cercariae of Schistosoma japonicum induced a high level of humoral immune response but with a low level of protection, suggesting that C57BL / 6 mice are low-responding strains of UV-attenuated Schistosoma japonicum cercariae.