Quercetin promotes neurite growth through enhancing intracellular cAMP level and GAP-43 expression

• 作 者: CHEN Ming-Ming YIN Zhi-Qi ZHANG Lu-Yong LIAO Hong
• 机 构: National Center for Drug Screening, China Pharmaceutical University,Department of Phytochemistry, Ch
• 出 处: Chinese Journal of Natural Medicines
• 发表日期: 2015年09期
• 关键词: quercetin intracellular manner conclusion length enhancing intrinsic activating

The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E-115 cells in vitro by use of microscopy, LANCE? cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. The Gap-43 expression was up-regulated in a time-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular c AMP level and Gap-43 expression.

The present study was designed to investigate the role of quercetin on neurite growth in N1E-115 cells and the underlying mechanisms. Quercetin was evaluated for its effects on cell numbers of neurites, neurite length, intracellular cAMP content, and Gap-43 expression in N1E -115 cells in vitro by use of microscopy, LANCE® cAMP 384 kit, and Western blot analysis, respectively. Our results showed that quercetin could increase the neurite length in a concentration-dependent manner, but had no effect on the numbers of cells. Quercetin significantly increased the expression of cellular cAMP in a time- and concentration-dependent manner. In conclusion, quercetin could promote neurite growth through increasing the intracellular c AMP level and Gap-43 expression.