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目的:探讨人类白细胞抗原B*27(HLA-B*27)及其亚型与强直性脊柱炎(AS)患者发病的相关关系。方法:采用二重Taqman实时PCR方法检测23名AS就诊患者及其105名一级亲属的HLA-B*27,并采用高分辨的PCR-SSP技术分析HLA-B*27的亚型。结果:AS就诊患者HLA-B*27的阳性率为91.30%,其一级亲属HLA-B*27的阳性率为42.85%,患病率22.86%;3个家系的HLA-B*27亚型为HLA-B*2705,其他家系均为HLA-B*2704。结论:AS的发生与HLA-B*27密切相关,且具有家族遗传性;与HLA-B*2705比较,HLA-B*2704是发生AS更危险的因素。
Objective: To investigate the relationship between human leukocyte antigen B * 27 (HLA-B * 27) and its subtypes in patients with ankylosing spondylitis (AS). Methods: HLA-B * 27 was detected by double Taqman real-time PCR in 23 patients with AS and its 105 first-degree relatives. HLA-B * 27 subtypes were analyzed by high resolution PCR-SSP. Results: The positive rate of HLA-B * 27 in AS patients was 91.30%, the positive rate of HLA-B * 27 in first-degree relatives was 42.85%, the prevalence was 22.86%. The HLA-B * 27 subtypes For HLA-B * 2705, all other families are HLA-B * 2704. CONCLUSIONS: The occurrence of AS is closely related to HLA-B * 27 and has familial inheritance. Compared with HLA-B * 2705, HLA-B * 2704 is a more dangerous factor in the development of AS.