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采用荧光和UV光谱法研究了盐酸药根碱(Jat)与牛血清白蛋白(BSA)相互作用的光谱特性。结果表明:Jat对于BSA荧光猝灭主要是静态猝灭和非辐射能量转移;Jat浓度增大,BSA荧光峰被猝灭的同时出现峰裂分现象,原来345 nm处的单峰逐渐裂分为二重峰,其中一峰峰值蓝移,另一峰峰值红移至λem=362～365 nm;测得不同温度下的结合常数及结合位点数;热力学常数探讨作用机理,主要以疏水作用力与BSA相互作用;作用过程是一个熵增加、G ibbs自由能降低的自发超分子作用过程。同步荧光技术研究了Jat对BSA构象的影响,表明BSA荧光主要源于色氨酸残基,Jat对BSA的构象均有影响。F rster偶极-偶极非辐射能量转移理论,计算了Jat与BSA作用距离<7 nm。
The spectral characteristics of the interaction between Jat and bovine serum albumin (BSA) were studied by fluorescence and UV spectroscopy. The results showed that: Jat fluorescence quenching of BSA mainly static quenching and non-radiative energy transfer; Jat concentration increases, BSA fluorescence peak was quenched at the same time the phenomenon of peak splitting, the original single peak at 345 nm gradually split into Double peak, one of the peak blue-shift, and the other peak red-shifted to λem = 362 ~ 365 nm; measured under different temperature binding constants and binding sites; thermodynamic constants to explore the mechanism of action mainly hydrophobic interaction with BSA Role; role of the process is an increase in entropy, G ibbs free energy to reduce spontaneous supramolecular processes. The effect of Jat on the conformation of BSA was studied by synchronous fluorescence technique, indicating that BSA fluorescence mainly originated from tryptophan residues and that Jat had an influence on the conformation of BSA. F rster dipole-dipole non-radiative energy transfer theory, the interaction distance between Jat and BSA is calculated to be less than 7 nm.