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本文报告测定人外周血抗原特异性B细胞前体频率的有限稀释分析法。以TT免疫健康个体,其外周血单个核细胞在PWM刺激下做系列稀释的多复孔(n=10)培养,8天后用间接ELISA方法测定上请中特异性抗体。阴性阈值定义为无细胞培养液OD值与其二倍标准差之和。在此条件下,阴性孔率的
This article reports a limited dilution assay for determining the frequency of antigen-specific B cell precursors in human peripheral blood. Individuals immunized with TT were cultured in serial dilutions (n = 10) of peripheral blood mononuclear cells under the stimulation of PWM. After 8 days, the specific antibodies were detected by indirect ELISA. The negative threshold is defined as the OD of the cell-free culture plus the double standard deviation. In this condition, the negative rate of porosity