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目的研究心脏正常发育和异常分化过程中磷酸化p38(p-p38)的表达变化,探讨p38在高温处理后金黄地鼠胚胎心脏发育中的作用。方法将金黄地鼠孕鼠于受孕8d置42℃水浴持续20min,于高温处理后12h、24h、36h、48h剖腹取胎,制备石蜡切片。利用免疫荧光染色技术,观察心脏正常发育和异常分化过程中磷酸化p38(p-p38)的表达变化。结果 p-p38的免疫阳性产物表达于鼠胚内皮细胞及心胶质细胞胞浆中,高温处理后不同时间点实验组p-p38的表达均较对照组明显降低。结论高温可导致金黄地鼠胚胎心脏p-P38表达减弱,p-p38对心脏的正常发育具有重要作用。
Objective To study the expression of phosphorylated p38 (p-p38) during normal cardiac development and abnormal differentiation, and to explore the role of p38 in the embryonic heart development of golden hamster. Methods The pregnant mice of golden hamster were immersed in 42 ℃ water bath for 8 days and 20 ℃ for 12h, 24h, 36h and 48h after pyrogen treatment. Paraffin sections were prepared. Immunofluorescence staining was used to observe the expression of phosphorylated p38 (p-p38) during normal cardiac development and abnormal differentiation. Results The expression of p-p38 immunopositive product was found in the cytoplasm of rat embryonic endothelium and cardiac glia. The expression of p-p38 in experimental group was significantly lower than that in control group at different time points after high temperature treatment. Conclusion High temperature can lead to the weakened expression of p-P38 in the heart of golden hamster, and p-p38 plays an important role in the normal development of the heart.