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目的 研究人外周血淋巴细胞在不同冻存条件下保存不同时间后DNA的损伤程度 ,从而评价SCGE最适宜的细胞保存方法和时间。方法 将未处理的和H2 O2 处理的人外周血淋巴细胞分别储存于 4℃、-2 0℃和 -70℃ ,于保存 0h、 2 4h、 48h、 72h、 96h、 192h后取出 ,进行单细胞凝胶电泳 (SCGE)试验。结果 未处理的和经H2 O2 处理的人外周血淋巴细胞在不同冰冻条件 ( 4℃、 -2 0℃、 -70℃ )下 ,保存一定的时间 ( 2 4h、 48h、 72h、 96h、 192h)后 ,经电泳表明淋巴细胞DNA的迁移长度和DNA拖尾率结果比较差异有显著意义。结论 4℃情况下保存细胞以 2 4h内为佳 ,保存更长时间需 -70℃。
Objective To study the DNA damage of human peripheral blood lymphocytes stored at different time under different cryopreservation conditions so as to evaluate the most suitable cell preservation method and time for SCGE. Methods Untreated and H2O2-treated human peripheral blood lymphocytes were stored at 4 ℃, -2 ℃ and -70 ℃, respectively, and stored for 0h, 24h, 48h, 72h, 96h and 192h, Gel electrophoresis (SCGE) test. Results Untreated and H2O2-treated human peripheral blood lymphocytes were stored for a certain period of time (24 h, 48 h, 72 h, 96 h, 192 h) under different freezing conditions (4 ° C, -2 ° C, -70 ° C) After electrophoresis showed that the lymphocyte DNA migration length and DNA tailing rate results were significant differences. CONCLUSIONS: Preservation of cells at 4 ° C is preferred within 24 hours and should be stored at -70 ° C for longer periods of time.