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目的:研究温阳化浊汤方对慢性非细菌性前列腺炎大鼠血清及前列腺组织中TNF-a、IL-6、IL-8影响。方法:健康雄性SD大鼠40只,体重500~800g,随机均分为4组,空白组、模型组、中药组(温阳化浊汤4号),阳性对照(莫西沙星)组。采用去势术后皮下注射苯甲酸雌二醇0.25mg/(kg·d),30 d建立慢性非细菌性前列腺炎模型。用温阳化浊汤4号灌胃,对照组莫西沙星,余均用生理盐水灌胃。连续治疗7 d。采用ELISA法检测大鼠血清和前列腺组织匀浆内TNF-α、IL-6、IL-8的表达。结果:温阳化浊汤方组血清和前列腺组织中TNF-α、IL-8、IL-6表达显著低于模型组(P<0.01),与阳性对照组没有显著差异。温阳化浊汤方与模型组前列腺内白细胞数明显减少(P<0.01)。结论:温阳化浊汤方对大鼠慢性非细菌性前列腺炎有一定治疗作用,其作用机制可能与降低血液及组织中细胞因子IL-8、TNF-α水平有关。
Objective: To investigate the effect of Wenyang Huazhuo Tang prescription on TNF-a, IL-6 and IL-8 in serum and prostate of chronic non-bacterial prostatitis rats. Methods: Forty healthy male Sprague Dawley rats weighing 500 ~ 800g were randomly divided into 4 groups: blank group, model group, TCM group (Wenyang Huazhuotang No.4) and positive control group (Moxifloxacin group). After castration, subcutaneous injection of estradiol benzoate 0.25mg / (kg · d), 30d chronic non-bacterial prostatitis model. With Yang Yang Huazhuo Decoction gavage on the 4th, the control group moxifloxacin, I were administered with saline. Continuous treatment for 7 days. The expression of TNF-α, IL-6 and IL-8 in serum and prostatic tissue were detected by ELISA. Results: The expressions of TNF-α, IL-8 and IL-6 in serum and prostatic tissue of Wenyang Huazhuo Decoction group were significantly lower than those in model group (P <0.01), but not significantly different from those in positive control group. Wenyang Huazhuo Decoction and model group, the number of leukocytes in the prostate decreased significantly (P <0.01). Conclusion: Wenyang Huazhuo Decoction has certain therapeutic effect on chronic nonbacterial prostatitis in rats, and its mechanism may be related to reducing the levels of cytokines IL-8 and TNF-α in blood and tissues.