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印度比哈尔邦内脏利什曼病十分流行。传统的解剖白蛉调查自然感染方法,效率低且无法检测轻度感染的白蛉。分子生物学技术为此提供了快捷、特异的方法。本文讨论了非放射性地高辛标记的杜氏利什曼原虫全虫DNA探针检测白蛉感染利什曼原虫的情况。
Visceral leishmaniasis is very prevalent in Bihar, India. Traditional anatomical whiteflies investigate natural infection methods, which are inefficient and unable to detect lightly infected whiteflies. Molecular biology techniques provide a quick and specific method for this purpose. This article discusses the detection of leishmaniasis in whitefly by non-radioactive digoxigenin-labeled Leishmania donovani DNA probe.