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在水温10～12℃条件下,研究了仿刺参幼参暴露于Cu2+浓度为0.010 mg/L、0.012 mg/L和0.050 mg/L的海水中75 d,对其生长和存活的影响及对Cu2+的蓄积情况。结果表明,Cu2+浓度为0.010 mg/L和0.012 mg/L组中幼参未发生中毒现象,存活率均为100%。前者体长、体重增长率与对照组差异不显著(P>0.05);而后者体长增长率与对照组差异不显著(P>0.05),但体重增长率与对照组差异显著(P<0.05);0.050 mg/L组幼参表现出中毒症状,存活率仅为(65.33±8.33)%。体长、体重增长率很低。前两组幼参对Cu2+的蓄积均表现为先积累后降低的趋势,实验结束时蓄积量和蓄积速率趋近于对照组(P>0.05);0.050 mg/L组蓄积量随着暴露时间延长持续升高,表观上陆续出现中毒及死亡的现象,表明此时Cu2+浓度已经超出幼参的代谢调节限度,Cu2+慢性胁迫对幼参生长和生存已产生严重影响。
The effects of exposure to sea cucumber seedlings exposed to Cu2 + at concentrations of 0.010 mg / L, 0.012 mg / L and 0.050 mg / L for 75 d on the growth and survival were studied at the water temperature of 10-12 ℃. Cu2 + accumulation situation. The results showed that the juveniles were not poisoned when the concentrations of Cu2 + were 0.010 mg / L and 0.012 mg / L, and the survival rate was 100%. The body length and body weight growth rate were not significantly different from the control group (P> 0.05), while the latter body length growth rate was not significantly different from the control group (P> 0.05), but the body weight growth rate was significantly different from that of the control group ). The young rats in 0.050 mg / L group showed symptoms of poisoning with survival rate of (65.33 ± 8.33)%. Body length, weight gain rate is low. The accumulation of Cu2 + in the first two groups showed the trend of first accumulation and then decrease, the accumulation and accumulation rate approached to the control group at the end of the experiment (P> 0.05); the accumulation of 0.050 mg / L group increased with the extension of exposure time Continued to rise, the appearance of successive poisoning and death phenomenon, indicating that at this time Cu2 concentration has exceeded the metabolic regulation of young ginseng, Cu2 + chronic stress on the growth and survival of the ginseng have a serious impact.