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目的:探讨宫颈癌中高危型HPV病毒E 6基因、P 73基因在CIN及宫颈癌中的表达及其相关性,为宫颈癌的临床诊断及开展基因治疗提供实验依据。方法:采用定量RT-PCR技术检测研究40例宫颈浸润癌组织、30例宫颈上皮内瘤变、20例慢性宫颈炎组织、30例正常宫颈组织中P 73基因及高危型HPV病毒E 6基因的表达。结果:P 73基因及高危型HPV病毒E 6基因在宫颈癌中阳性率分别为87.50%、95.00%,在宫颈上皮内瘤变中阳性率分别76.67%,70.00%,在慢性宫颈炎中阳性率分别为55.00%、20.00%,在正常宫颈组织中阳性率分别为20.00%、0.00%,差异有统计学意义(P<0.05)。结论:P 73基因表达、高危型HPV病毒E 6基因与宫颈癌的发生、发展有关。
Objective: To investigate the expression and correlation of E 6 gene and P 73 gene of cervical cancer in high-risk HPV type C and cervical cancer and to provide experimental evidence for the clinical diagnosis and gene therapy of cervical cancer. Methods: Quantitative RT-PCR technique was used to detect the expression of P 73 gene and high-risk HPV E 6 gene in 40 cases of invasive cervical carcinoma, 30 cases of cervical intraepithelial neoplasia, 20 cases of chronic cervicitis and 30 cases of normal cervix. expression. Results: The positive rates of P 73 gene and high-risk HPV E 6 gene in cervical cancer were 87.50% and 95.00%, respectively. The positive rates of P 73 gene and cervical intraepithelial neoplasia were 76.67% and 70.00% Respectively, 55.00% and 20.00% respectively. The positive rates in normal cervical tissues were 20.00% and 0.00%, respectively, with significant difference (P <0.05). Conclusion: The expression of P 73 gene and the high-risk HPV 6 E6 gene are associated with the occurrence and development of cervical cancer.