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初步探讨APPJ等离子体对微生物的影响机制 ,用APPJ与DBD两种不同类型等离子体对不同代表微生物金黄色葡萄球菌、大肠杆菌、枯草杆菌黑色变种进行处理 ,分析比较不同微生物对不同等离子体的存活曲线 ;进而利用扫描电镜观察微生物细胞壁、膜等外部结构的变化。结果显示两种等离子体对不同微生物的杀灭作用均为先快后慢 ,APPJ的作用效果远好于DBD(DBD对金黄色葡萄球菌及枯草杆菌黑色变种芽孢的D值为 70s ,而APPJ的D值为 4s)。同时 ,在APPJ的作用下 ,大肠杆菌细胞壁、膜有明显破裂发生。这证明 ,APPJ可快速有效地杀灭微生物体 ,其灭菌机制可能与微生物细胞壁、膜的破裂有关。
The effects of APPJ plasma on microorganisms were investigated. Different APPM and DBD plasmas were used to treat different representative strains of Staphylococcus aureus, Escherichia coli and Bacillus subtilis. The survival of different plasmas against different plasmas was analyzed Curve; and then use scanning electron microscopy to observe changes in microbial cell wall, membrane and other external structures. The results showed that the killing effect of the two kinds of plasma on different microorganisms was fast and slow, the effect of APPJ was much better than that of DBD (the D value of DBD for Staphylococcus aureus and Bacillus subtilis black spores was 70s, while APPJ D value of 4s). At the same time, under the action of APPJ, E. coli cell wall, membrane rupture occurred. This proves that APPJ can kill microorganisms rapidly and effectively. The sterilization mechanism may be related to the cell wall and membrane rupture.