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运用Grimelius银染法对菲牛蛭(Poecilobdella manillensis)消化管内嗜银细胞的分布和形态进行观察,并根据嗜银细胞的分布特点统计其分布密度。消化管除食道外各部位均有嗜银细胞分布,细胞染棕色或黑色;嗜银细胞基本位于上皮细胞之间和固有层内,主要呈圆形、椭圆形、锥体形和长条形等多种形态;部分细胞胞突明显,细胞内可见黑色分泌颗粒。嗜银细胞分布密度为颚片最高,嗉囊次之,直肠最低,食道内未检测出嗜银细胞。消化管各段嗜银细胞的形态特征和分布规律可能与其食性和消化生理活动密切相关。
The distribution and morphology of argyrophilic cells in the digestive tract of Poecilobdella manillensis were observed by Grimelius silver staining, and the distribution density of argyrophil cells was calculated according to the distribution characteristics of argyrophilic cells. In addition to esophageal digestive tract in all parts of argyrophilic cells are distributed, cells stained brown or black; argyrophil cells located in the epithelial cells and lamina propria, mainly round, oval, pyramidal and long and many more Species; some cells prominent cell processes, cells can be seen within the black secretion of particles. The density of argyrophilic cells was the highest in the palate, followed by the carapace, the lowest in the rectum, and no argyrophilic cells were detected in the esophagus. Morphological characteristics and distribution of argyrophilic cells in various stages of the digestive tract may be closely related to their feeding habits and digestive and physiological activities.