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目的 研究雪旺细胞胞浆神经营养蛋白 (SDNF)对周围神经再生的影响。方法 选用 90只成年SD大鼠 ,在右侧坐骨神经造成长 10 m m缺损 ,用植入血管的变性骨骼肌桥接神经缺损。分成三组 ,每组 30只。分别将分子量为 2 6、5 8ku的 SDNF和生理盐水 (对照组 )于术中、术后 7及 14天注入肌桥的近段、中段和远段。术后均观察 6个月。术后 15天开始 ,每隔 15天走足印一次 ,测定坐骨神经功能指数。术后 1、3、6个月 ,每组取 10只大鼠行神经电生理检测、比目鱼肌收缩力测定和组织形态学观察。结果 分子量为 2 6、5 8ku的 SDNF两实验组坐骨神经功能恢复指数、神经电生理、比目鱼肌收缩力和形态学观察指标 ,与对照组比较有非常显著性差异 (P<0 .0 1)。结论 分子量为 2 6和 5 8ku的 SDNF有促进损伤的周围神经再生作用。
Objective To study the effect of Schwann cell cytoplasmic neurotrophic protein (SDNF) on the peripheral nerve regeneration. Methods Ninety adult Sprague-Dawley rats were randomly divided into two groups. The right sciatic nerve resulted in a 10-m-long defect, and the nerve defects were implanted into the degenerative skeletal muscle. Divided into three groups of 30. SDNF and saline (control group) with a molecular weight of 26,58 ku were injected intraoperatively into the proximal, middle and distal segments of the muscle bridge at 7 and 14 days after operation. All patients were observed for 6 months. Begin 15 days after operation and walk every 15 days to determine the sciatic nerve function index. At 1, 3 and 6 months after operation, 10 rats in each group were subjected to electrophysiological examination, contractile force measurement and histomorphology of soleus muscle. Results The function recovery index, nerve electrophysiology, soleus muscle contractility and morphological observation index of SDNF two groups with molecular weight of 26,58ku were significantly different (P <0.01) compared with the control group. Conclusion SDNFs with molecular weights of 26 and 58 kDa have the effect of promoting peripheral nerve regeneration after injury.