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平滑肌细胞(SMC)增生在动脉粥样硬化(AS)病变发生过程中起关键作用,增生的 SMC 由收缩型转变成合成型,分泌大量细胞外基质。而 c—sis 和 c—myc 基因在控制细胞增生过程中起重要作用。本文用异羟基洋地黄毒甙配基—durp 为底物,随机插入法进行 DNA 标记,通过酶联免疫斑点杂交法检测了胎儿(10例)和成人(6例)主动脉中膜、动脉硬化斑块和斑块旁中膜(6例)
Smooth muscle cell (SMC) hyperplasia plays a key role in the pathogenesis of atherosclerosis (AS), and hyperplastic SMCs transform from contractile to synthetic and secrete large amounts of extracellular matrix. The c-sis and c-myc genes play an important role in the control of cell proliferation. In this study, digoxigenin-durp was used as a substrate for DNA labeling by random insertion method. The aortic tunica intima and atherosclerosis were detected in fetal (10 cases) and adult (6 cases) by enzyme-linked immunosorbent assay (FISH) Plaque and plaque next to the media (6 cases)