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目的探讨呼吸道合胞病毒(respiratory syncytial virus,RSV)感染后,使用广谱抗生素头孢哌酮引起的咽部优势菌群清除对其气道炎症、气道高反应,以及对肺部CD11c+细胞状态的影响。方法 3周龄雌性Balb/c随机分入空白对照组、RSV感染组和RSV感染后口服头孢哌酮组。7 d后检测口服头孢哌酮对咽部优势菌群的清除作用,并对各组小鼠进行肺泡灌洗液炎症细胞分类计数、病理切片HE染色及气道高反应性检测,流式细胞术检测肺部CD11c+细胞MHC-II及共刺激分子表达。结果 1)正常小鼠咽部优势菌群为链球菌,RSV感染未影响咽部优势菌群的改变,口服头孢哌酮可有效清除链球菌;2)RSV感染后急性期支气管肺泡灌洗液中炎症细胞明显增多[(13.20±1.72)×105 vs(6.17±0.28)×105 cells/ml,P<0.05],肺部病理损伤加重,气道高反应性轻微上升,肺部CD11c+细胞上MHC-II表达较空白对照明显上调(平均荧光强度:14 205±1 519 vs 9 707±1 140,P<0.05);头孢哌酮致咽部优势菌的清除未进一步加重气道炎症及气道高反应性,但在一定程度上抑制了CD11c+细胞的成熟,表现为单个细胞表面MHC-II表达水平较单独感染RSV组降低,与空白对照无明显差异。结论 Balb/c小鼠感染RSV同时使用广谱抗生素头孢哌酮导致咽部优势菌被清除,可能减少抗原对肺部CD11c+细胞刺激,从而一定程度上影响CD11c+细胞的成熟。
Objective To investigate the effects of clearance of pharyngeal dominant bacteria caused by respiratory syncytial virus (RSV) on airway inflammation, airway hyperresponsiveness and lung CD11c + status influences. Methods Female Balb / c at 3 weeks old were randomly divided into blank control group, RSV infection group and oral cefoperazone group after RSV infection. 7 days after oral cefoperazone on pharyngeal dominant bacteria scavenging effect, and the mice in each group were alveolar lavage fluid inflammatory cell count, pathological sections HE staining and airway hyperresponsiveness detection, flow cytometry The expressions of MHC-II and costimulatory molecules on CD11c + cells in lung were detected. Results 1) The predominant pharyngeal flora in normal mice was Streptococcus, RSV infection did not affect the dominant pharyngeal flora, oral administration of cefoperazone can effectively remove Streptococcus; 2) RSV infection in the acute bronchoalveolar lavage fluid (13.20 ± 1.72) × 105 vs (6.17 ± 0.28) × 105 cells / ml, P <0.05]. The pathological injury of lung increased and the airway hyperresponsiveness increased slightly. The expression of MHC- II expression was significantly upregulated compared with the blank control (mean fluorescence intensity: 14 205 ± 1 519 vs 9 707 ± 1 140, P <0.05). The clearance of cefoperazone-induced pharyngogenic bacteria did not further aggravate airway inflammation and airway hyperresponsiveness But inhibited the maturation of CD11c + cells to a certain extent. The expression of MHC-II on single cell surface was lower than that of RSV alone, and there was no significant difference with blank control. Conclusion The infection of RSV with Balb / c mice resulted in the elimination of dominant phagofloxacin from the broad-spectrum antibiotic Cefoperazone, which may reduce the stimulation of CD11c + cells by antigens in lungs and thus affect the maturation of CD11c + cells to a certain extent.