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目的:探讨菊花总黄酮对小儿RSV感染A549细胞诱导RANTES及MCP-1释放作用影响。方法:实验分为细胞对照组,病毒对照组,菊花总黄酮组和病毒唑组。在Hep-2细胞和A549细胞分别加入菊花总黄酮和病毒唑的含药维持液,测定上述两种药物的最大无毒浓度;RSV病毒感染Hep-2细胞,观察药物对RSV的病毒抑制作用;RSV感染A549细胞,ELISA法测细胞趋化因子RANTES及MCP-1含量。结果:菊花总黄酮50%有效率优于病毒唑组,差异有统计学意义(P<0.05);RANTES及MCP-1释放抑制作用比较中,菊花总黄酮组RANTES、MCP-1明显降低,优于病毒唑组,差异有统计学意义(P<0.05)。结论:菊花总黄酮能够抑制RSV病毒活性,明显降低A549细胞释放RANTES、MCP-1,缓解患儿的呼吸道症状,对临床具有指导意义,值得临床推广。
Objective: To investigate the effects of chrysanthemum flavonoids on the release of RANTES and MCP-1 in children with RSV-infected A549 cells. Methods: The experiment was divided into cell control group, virus control group, chrysanthemum total flavone group and ribavirin group. RSV was used to infect Hep-2 cells and A549 cells respectively. The RSV virus was used to infect Hep-2 cells to observe the virus inhibitory effect of RSV on Hep-2 cells and A549 cells. A549 cells were infected with RSV, and the chemotactic factors RANTES and MCP-1 were assayed by ELISA. Results: The effective rate of 50% of the total flavonoids of chrysanthemum was better than that of ribavirin (P <0.05). Compared with the release inhibition of RANTES and MCP-1, RANTES and MCP-1 of the total flavonoids of chrysanthemum significantly decreased In ribavirin group, the difference was statistically significant (P <0.05). Conclusion: The total flavonoids of chrysanthemum can inhibit the activity of RSV and significantly reduce the release of RANTES and MCP-1 in A549 cells, which can relieve the respiratory symptoms of children. It is of clinical significance and worthy of clinical promotion.