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目的:证明NDV D90是否具有抑制口腔鳞癌细胞系迁移和侵袭的能力。方法:免疫荧光染色法检测D90对细胞微管和微丝形态的改变;Transwell法检测D90对HN-6细胞迁移和侵袭率的抑制作用;蛋白印迹法检测D90对于SP1、RECK、MMP-2和MMP-9表达的影响;明胶酶谱法用于检测MMP-2和MMP-9活性的改变。结果:实验结果表明,D90通过改变细胞的微管和微丝形态来抑制细胞的能动性;D90具有抑制HN-6细胞的迁移和侵袭的功能。同时,D90通过下调SP1和上调RECK的表达来抑制MMP-2和MMP-9的表达和活性。结论:NDV D90能够有效地抑制口腔鳞癌细胞系HN-6的迁移和侵袭,为一种新的抗肿瘤制剂提供了临床试验基础。
OBJECTIVE: To demonstrate whether NDV D90 has the ability to inhibit the migration and invasion of oral squamous cell carcinoma cell lines. METHODS: Immunofluorescence staining was used to detect the morphological changes of microtubules and microfilaments in D90 cells. Transwell assay was used to detect the inhibitory effect of D90 on the migration and invasion of HN-6 cells. Western blotting was used to detect the expression of SP1, RECK, MMP-2 and MMP-9 expression; gelatin zymography was used to detect the changes of MMP-2 and MMP-9 activity. Results: The experimental results showed that D90 inhibited the motility of the cells by changing the morphology of microtubules and microfilaments. D90 also inhibited the migration and invasion of HN-6 cells. In the meantime, D90 inhibits the expression and activity of MMP-2 and MMP-9 by down-regulating SP1 and up-regulating RECK expression. Conclusion: NDV D90 can effectively inhibit the migration and invasion of oral squamous cell carcinoma cell line HN-6, providing the basis for clinical trial of a new anti-tumor agent.