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Radular teeth of chitons were studied by using magnetic torque-meter and transmission electron microscopy (TEM). The magnetic torque curves give clear evidence of presence of strong uni-axial magnetic anisotropy. The easy axis is along the length direction of tongue-like radula. The TEM patte shows that long chip-like magnetite nano-scaled particles packed in the radular teeth with both uni-axial shape anisotropy and magneto-crystalline anisotropy.