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本实验用15μg鹅膏蕈氨酸分3点注入SD大鼠右侧尾壳核头部。术后1个月进行跳台试验,有29只动物(占被破坏鼠69％)表现明显的学习记忆能力低下,且存活7个月无明显变化。光电箱试验表明,大鼠有明显的夜间过度活动。形态学观察发现,破坏后1个月鼠尾壳核头部萎缩,侧脑室扩大,破坏区神经元明显减少,胶质增生。破坏后7个月,破坏区乙酰胆碱酯酶(AChE)组织化学染色阳性细胞以及胆碱乙酰化酶(ChAT)、γ-氨基丁酸(GABA)免疫组织化学染色阳性细胞明显减少,破坏侧苍白球、黑质网状部及覆盖破坏区的皮质萎缩。结果表明该动物模型是成立的。
In this experiment, 15 μg of ibotenic acid was injected into the head of the caudate putamen on the right of SD rats at 3 points. Jump test was performed 1 month after operation. Twenty-nine animals (69% of the destroyed rats) showed obvious learning and memory abilities, and no significant changes were found after 7 months of survival. Photoelectric box test showed that rats have obvious nighttime over-activity. Morphological observation found that 1 month after destruction, the head of rat caudate putamen atrophy, enlargement of the lateral ventricle, neurons in the destruction of significantly reduced glial proliferation. Seven months after vandal injury, positive cells of AChE histochemical staining and ChAT, GABA immunohistochemical staining of positive cells were significantly reduced, which destroyed the lateral globus pallidus , The substantia nigra mesh and cover the destruction of cortical atrophy. The results show that the animal model is established.