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目的研究氟在大鼠脑组织中的蓄积及其对脂质过氧化水平和抗氧化能力的影响。方法用氟化钠对大鼠进行亚慢性染毒,测定大鼠脑组织中氟浓度和脂质过氧化产物丙二醛(MDA)的含量及抗氧化酶超氧化物歧化酶(SOD)、谷胱甘肽过氧化物氧化酶(GSH-Px)的活力。结果大鼠脑组织中氟浓度与染毒剂量呈正相关,高剂量组脑组织氟浓度明显高于低剂量组和对照组(P<0.01)。脂质过氧化产物MDA含量在三组间差异有统计学意义(P<0.01),高剂量组MDA含量高于低剂量组,低剂量组高于对照组。抗氧化酶SOD和GSH-Px的活力三组间差异有统计学意义(P<0.01)。但在组间比较时发现,低剂量组与对照组间差异没有统计学意义(P>0.05)。高剂量组与低剂量组、对照组间差异有统计学意义(P<0.01)。脑组织中氟浓度与MDA含量之间呈明显正相关(r=0.731,P<0.01),而与SOD和GSH-Px的相关性不明显(r分别为0.234和0.148,P>0.05),MDA与SOD和GSH-Px呈明显负相关(r分别为-0.571和0.591,P<0.01)。结论氟可透过血脑屏障在大鼠脑内蓄积。过量的氟接触将导致机体抗氧化系统紊乱,脂质过氧化程度加剧,造成脑组织氧化性损伤。
Objective To study the accumulation of fluoride in rat brain and its effect on lipid peroxidation and antioxidant capacity. Methods Rats were sub-chronically exposed to sodium fluoride. The levels of fluoride and the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in rat brain were measured. The activity of glutathione peroxidase oxidase (GSH-Px). Results The concentration of fluoride in the brain tissue of rats was positively correlated with the exposure dose. The concentration of fluoride in the high-dose group was significantly higher than that of the low-dose group and the control group (P <0.01). The content of MDA in lipid peroxidation products was significantly different among the three groups (P <0.01). The content of MDA in the high dose group was higher than that in the low dose group, while that in the low dose group was higher than that in the control group. The activities of antioxidant enzymes SOD and GSH-Px were significantly different among the three groups (P <0.01). However, when compared between groups, there was no significant difference between the low-dose group and the control group (P> 0.05). There was significant difference between high dose group and low dose group and control group (P <0.01). There was a significant positive correlation between fluoride content and MDA content in brain tissue (r = 0.731, P <0.01), but not with SOD and GSH-Px (r = 0.234 and 0.148, P> 0.05) Negatively correlated with SOD and GSH-Px (r = -0.571 and 0.591, respectively, P <0.01). Conclusion Fluorine can accumulate in rat brain through the blood-brain barrier. Excessive fluoride exposure will cause the body’s antioxidant system disorders, increased levels of lipid peroxidation, resulting in oxidative damage of brain tissue.