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依曲替酯(Ro 10-9359)是一种广泛用于银屑病治疗的芳香维甲酸药物,口服后在体内被水解成有活性的依曲替酸(R0 10-1670)。已有报道维甲酸可影响免疫反应、炎症反应及表皮的脂质合成,但其对脂肪酸代谢的作用所知甚少。由于脂质合成和脂肪酸代谢的变化明显影响多种细胞功能,作者以~(14)C 标记法探讨了依曲替酸对体外培养的人角朊细胞花生四烯酸的掺入、分布及释放所超的作用,并分析了这些细胞的脂肪酸组成。结果发现,在24小时放射标记培养期内,培养的角朊细胞不断摄取~(14)C 标记的花生四烯酸,约有85.3%±0.2%的放射性掺入细胞。标记后,角朊细胞在加入不同
Etrotritol (Ro 10-9359) is an aromatic retinoid that is widely used in the treatment of psoriasis and is hydrolyzed in vivo to the active iitatisilicate (R0 10-1670). Retinoic acid has been reported to affect immune responses, inflammatory responses and epidermal lipid synthesis, but little is known about its effect on fatty acid metabolism. Due to changes in lipid synthesis and fatty acid metabolism significantly affect a variety of cell functions, the authors discussed the incorporation, distribution and release of ettringite for arachidonic acid cultured human keratinocytes by ~ (14) C labeling The role of superfluous, and analyzed the fatty acid composition of these cells. The results showed that cultured keratinocytes continuously uptake of ~ (14) C-labeled arachidonic acid and about 85.3% ± 0.2% of radioactively incorporated cells during the 24-hour radiolabeled culture. After labeling, keratinocytes are added differently