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由于人二倍体细胞狂犬疫苗价格较高而使其应用受到限制,特别是在对狂犬疫苗需求量很大的发展中国家更是如此。因此,研制出一种便宜得多的,以鸭胚繁殖病毒而制备的疫苗。新疫苗是由Pitman—Moore株狂犬病毒制备的,在将病毒自鸭胚组织提取出来作成病毒悬液后,经过滤、沉淀、离析加以提纯、浓缩后,再以β—Propiolactone灭活,并由此制备出一种高纯度的鸭胚疫苗,这种疫苗已除掉99%以上与抗原无关的蛋白质,故可减少异常反应。通过大鼠保护试验证明,该疫苗每剂抗原含量在2.5IU以上,此疫苗除了占有
Applications are limited due to the high price of human diploid rabies vaccines, especially in developing countries where rabies vaccines are in high demand. Thus, a much cheaper vaccine has been developed that reproduces the virus with duck embryos. The new vaccine is prepared from Pitman-Moore strains of rabies virus. After the virus is extracted from the duck embryo tissue and made into a virus suspension, the virus is purified by filtration, sedimentation and isolation, concentrated, and then inactivated by β-Propiolactone. This prepared a high purity duck embryo vaccine, this vaccine has been removed more than 99% of the antigen has nothing to do with the protein, it can reduce the abnormal reaction. Through rat protection test proved that the vaccine antigen dose per dose above 2.5IU, the vaccine in addition to possession