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为研究杨树多样性和不同分子标记技术间的差异与联系,采用SSR和ITS标记分析12个杨树样品的DNA多态性,并在遗传距离的基础上对这两种标记方法进行相关性分析。SSR标记表明,13对引物共扩增条带54条,多态性条带51条,PIC值为0.36~0.76,能较好的区分不同样品。ITS分析结果显示,供试杨树之间差异不仅表现在碱基的差异,也表现在序列长度的差异。利用这两种标记方法对所有样品进行聚类分析发现,二者在派别间分类基本一致,但在细微分类中SSR标记相对于ITS技术能更准确的鉴别出来源相同或相似的植株个体。两种标记方法相关性分析表明,二者显著相关,相关系数R达到0.593(P=0.01,n=66,rα=0.314 966)。由此可见,它们均可用作杨树品种鉴定,同时结合这两种分子标记的遗传差异,可为快速准确的鉴定杨树资源提供技术依据。
In order to study the differences and relationships between poplar diversity and different molecular markers, SSR and ITS markers were used to analyze the DNA polymorphism of 12 poplar samples. Based on the genetic distance, the correlation between these two markers analysis. The SSR markers showed that 13 pairs of primers amplified 54 bands and 51 polymorphic bands with PIC values ranging from 0.36 to 0.76, which could distinguish different samples well. The results of ITS analysis showed that the differences between the tested poplars not only reflected differences in bases but also differences in sequence length. The clustering analysis of all the samples using these two markers showed that the two groups were basically the same among the factions. However, SSR markers could identify more accurately the same or similar plant individuals relative to ITS in subtle classification. Correlation analysis of the two markers showed that the two were significantly correlated, with a correlation coefficient R of 0.593 (P = 0.01, n = 66, rα = 0.314 966). Thus, they can be used as poplar species identification, combined with the genetic differences between the two molecular markers, can provide a technical basis for rapid and accurate identification of poplar resources.