高效液相色谱法测定风湿痛宁药酒中胡椒碱的含量

• 作 者: 裴妙荣 闫志芳 梁宏
• 机 构: 山西中医学院,山西省药品检验所
• 出 处: 中国中药杂志
• 发表日期: 1998年07期
• 关键词: 胡椒碱 高效液相色谱 精密度试验 线性范围 内标溶液 峰面积积分值 流动相 摇匀 稳定性试验 质量控制

目的：测定风湿痛宁药酒中胡椒碱的含量。方法：ＨＰＬＣ法。色谱条件为ＯＤＳ柱（１５０ｍｍ×４．６ｍｍ，５μｍ），流动相甲醇水（７３∶２７），检测波长３４３ｎｍ，柱温３０℃，流速０８ｍｌ／ｍｉｎ。结果：Ａｒ／Ａｓ＝０５６５２Ｍｒ／Ｍｓ－００３１８，ｒ＝０９９９９，ｎ＝３，线性范围００３２μｇ～０２１６μｇ；精密度ＲＳＤ＝１９２％，ｎ＝５；稳定性ＲＳＤ＝１８３％，４ｈ内稳定；重复性ＲＳＤ＝１５０％，ｎ＝５；回收率＝９８８０％，ＲＳＤ＝３５７％，ｎ＝５；回收率＝９８８０％，ＲＳＤ＝３５７％，ｎ＝５。结论：方法简便、灵敏、准确，可供本品质量控制用。

Objective: To determine the content of piperine in Fengtutongning wine. Method: HPLC method. The chromatographic conditions were ODS column (150mm × 4.6mm, 5μm), mobile phase methanol-water (73:27), detection wavelength 343nm, column temperature 30 ℃, flow rate 0.8ml / min. The results showed that the linearity ranged from 0.322 μg to 0.226 μg for Ar / As = 05652Mr / Ms-00318, r = 09999, n = 3, RSD = 1.92%, n = 5, RSD = 183%, stable within 4h; Repeatability RSD = 150%, n = 5; Recovery = 9880%, RSD = 357%, n = 5; Recovery = 9880 %, RSD = 357%, n = 5. Conclusion: The method is simple, sensitive and accurate for the quality control of this product.