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目的：比较研究补阳还五汤和其有效部位组方抗脑缺血后ＤＮＤ 作用与脑组织能量代谢、ＮＯ 和ＮＯＳ 的关系。方法：采用沙鼠双侧颈总动脉夹闭脑缺血再灌注模型，于再灌注后４８ 小时取前脑皮质测定Ｎａ ＋Ｋ＋ＡＴＰ 酶、Ｃａ２ ＋ＡＴＰ 酶、Ｍｇ２ ＋ＡＴＰ 酶、乳酸、ＮＯ 和ＮＯＳ。结果：缺血再灌注后４８ 小时脑组织Ｎａ＋Ｋ＋ＡＴＰ酶、Ｃａ２ ＋ＡＴＰ 酶及Ｍｇ２ ＋ＡＴＰ 酶活性均降低，ＮＯ 含量和ＮＯＳ 活性降低，而乳酸含量无明显变化。补阳还五汤和有效部位组方ｉｐ 均可防止缺血再灌注４８ 小时后Ｎａ＋Ｋ＋ＡＴＰ 酶活性的降低，增加ＮＯ 含量，提高ＮＯＳ 活性，而有效部位组方还可增加缺血再灌注后Ｃａ２ ＋ＡＴＰ 酶和 Ｍｇ２ ＋ＡＴＰ 酶活性，且其升高ＮＯ 的作用强于补阳还五汤组。结论：脑缺血后迟发性神经元坏死与脑内能量代谢障碍和ＮＯ 的生成减少有一定的关系，补阳还五汤及其有效部位组方可能通过调节脑内能量代谢和ＮＯ 的生成而发挥抗脑缺血作用
OBJECTIVE: To compare the relationship between the effect of DND and the energy metabolism, NO, and NOS in brain after anti-cerebral ischemia in rats treated with Buyang Huanwu Decoction and its effective fraction. METHODS: The cerebral ischemic reperfusion model was clamped by bilateral common carotid arteries in gerbils. Na + K + ATPase, Ca 2 + ATPase, and Mg 2 + ATPase were measured in the forebrain cortex 48 hours after reperfusion. Lactic acid, NO and NOS. Results: The activities of Na+K+ATPase, Ca2+ATPase and Mg2+ ATPase in brain tissue were decreased 48 hours after ischemia and reperfusion. NO content and NOS activity were decreased, while the content of lactic acid was not changed significantly. Both Buyang Huanwu Decoction and its active site group ip can prevent the decrease of Na+-K+-ATPase activity, increase the NO content and increase NOS activity after 48 hours of ischemia-reperfusion, and the effective site group can also increase ischemia. Ca2 + ATPase and Mg2 + ATPase activity after infusion, and its role in increasing NO was stronger than Buyang Huanwu Decoction group. Conclusion: Delayed neuronal necrosis after cerebral ischemia is related to the decrease of energy metabolism and NO production in the brain. Buyang Huanwu Decoction and its active fractions may regulate energy metabolism and NO production in the brain. Anti-cerebral ischemia