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AIM:To investigate the prognostic significance of c-Kitgenemutation and DNA ploidy in gastointestinal stromal tumors(GISTs).METHODS:A total of 55 cases of GISTs were studied forthe expression of c-Kit by immunohistochemistry,and thec-Kitgene mutations in exons 9,11,13,and 17 weredetected by polymerase chain reaction-single strandconfirmation polymarphism(PCR-SSCP)and denaturinghigh performance liquid chromatography(D-HPLC)techniques.DNA ploidy was determined by flow cytometry.RESULTS:Of the 55 cases of GISTs,53 cases(96.4%)expressed c-Kitprotein.The c-Kitgene mutations of exons11 and 9 were found in 30(54.5%)and 7 cases(12.7%),respectively.No mutations were found in exons 13 and 17.DNA aneuploidy was seen in 10 cases(18.2%).The c-Kitmutation positive GISTs were larger in size than the negativeGISTs.The aneuploidy tumors were statistically associatedwith large size,high mitotic counts,high risk groups,highcellularity and severe nuclear atypia,and epithelioid type.There was a tendency that c-Kit mutations were morefrequently found in aneuploidy GISTs.CONCLUSION:DNA aneuploidy and c-Kit mutations canbe considered as prognostic factors in GISTs.
AIM: To investigate the prognostic significance of c-Kit gene mutation and DNA ploidy in gastointestinal stromal tumors (GISTs). METHODS: A total of 55 cases of GISTs were studied forthe expression of c-Kit by immunohistochemistry, and thec-Kitgene mutations in exons 9 , 11,13, and 17 weredetected by polymerase chain reaction-single strandconfirmation polymarphism (PCR-SSCP) and denaturing high performance liquid chromatography (D-HPLC) techniques. DNA ploidy was determined by flow cytometry.RESULTS: Of the 55 cases of GISTs, 53 cases (96.4%) expressed c-Kitprotein.Thec-Kitgene mutations of exons11 and 9 were found in30 (54.5%) and7 cases (12.7%), respectively.No mutations were found in exons13 and 17.DNA aneuploidy was seen in 10 cases (18.2%). The c-Kitmutation positive GISTs were larger in size than the negativeGISTs.The aneuploidy tumors were statistically associated with large size, high mitotic counts, high risk groups, highcellularity and severe nuclear atypia, and epithelioid type .There was a tendency th at c-Kit mutations were more frequently found in aneuploidy GISTs. CONCLUSION: DNA aneuploidy and c-Kit mutations canbe considered as prognostic factors in GISTs.