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作者报道在巨细胞病毒立即早期启动子控制下,构建了编码乙型肝炎病毒(HBV)的小(S)、中(S2+S)和大(S1+S2+S)包膜蛋白的质粒DNA表达载体,分别称为pCMV-S、pCMV-S1.S和pCMV-S1.S2.S.以pCMV-S 100μg对6～8周龄C57BL/6小鼠肌肉接种后1个月,产生的ELISA抗体滴度超过10~4,3个月时峰值为4×10~4左右,直到74周时仅稍有降低.如对成年小鼠(29周龄)免疫DNA,则抗体滴度峰值稍低(10~4),但至少可维持45周.此类成年小鼠,如以HBsAg蛋白免疫,则抗体滴度仅为10~2,以DNA免疫幼鼠和成鼠的抗体滴度分别相当於1000和100mIU/ml,成年小鼠以蛋白免疫者抗体滴度≤1mIU/ml.
The authors report that plasmid DNA encoding small (S), medium (S2 + S) and large (S1 + S2 + S) envelope proteins of Hepatitis B virus (HBV) was constructed under the control of the immediate early promoter of cytomegalovirus The expression vectors were respectively named pCMV-S, pCMV-S1.S and pCMV-S1.S2.S. One month after inoculation of pCMV-S 100 μg into muscle of 6 to 8 weeks old C57BL / 6 mice, the resulting ELISA Antibody titers of more than 10 to 4 at 3 and 4 weeks peaked at around 4 × 10 -4, with only a slight decrease at week 74. Immunization of DNA with adult mice (29 weeks of age) resulted in slightly lower antibody titers (10 ~ 4), but at least for 45 weeks.Such mice, such as HBsAg protein immunization, the antibody titers of only 10 ~ 2 to DNA immunization pups and adult mouse antibody titers equivalent to 1000 and 100mIU / ml, adult mice immunized with antibody titer ≤ 1mIU / ml.